Recombinant Human Leukemia Inhibitory Factor是參與各種神經和炎癥過程的淋巴因子，例如急性期反應，組織損傷和感染。
Synonyms
rHuLIF; Differentiation-stimulating Factor; D factor; MLPLI
Species
HumanSource
E. coli Accession
P15018 Gene ID
3976 Molecular Weight
Approximately 19.7 kDa AA Sequence
SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF Biological Activity
The ED50 is <0.2 ng/mL as measured by TF-I cells, corresponding to a specific activity of >5 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Leukemia Inhibitory Factor (rHuLIF) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Leukemia Inhibitory Factor (rHuLIF) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Leukemia Inhibitory Factor has been shown to possess a remarkable variety of actions. It releases calcium from bone tissue, and is the differentiation inhibitory factor preventing spontaneous differentiation in normal embryonic stem cells[1]. Leukemia inhibitory factor (LIF) is a neuropoietic cytokine involved in both the neural and immune responses to injury. Its levels are increased in a variety of animal and human inflammatory conditions. Administration of Leukemia Inhibitory Factor can suppress inflammatory signs in some cases, for instance after intratracheal lipopolysaccharide-induced inflammation. Leukemia Inhibitory Factor also increases corticosterone levels via the hypothalamo-pituitary-adrenal axis. Other evidence suggests, that it can also act as a proinflammatory cytokine. Exogenously added Leukemia Inhibitory Factor induces acute phase protein expression and stimulates the production of proinflammatory cytokines and monocyte chemoattractants[2]. |
