Recombinant Human Interleukin-6 有效誘導(dǎo)肝細(xì)胞合成急性期蛋白，參與炎癥反應(yīng)。
Synonyms
rHuIL-6; BSF-2; CDF; Hybridoma growth factor; IFN-beta-2
Species
HumanSource
E. coli Accession
P05231 Gene ID
3569 Molecular Weight
Approximately 21,000 Da AA Sequence
PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM Biological Activity
The ED50 is <0.1 ng/mL as measured by murine 7TD1 cells, corresponding to a specific activity of 5 × 107 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 20 mM HAc-NaAc, pH 5.0, 150 mM NaAc buffer. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Interleukin-6 (rHuIL-6) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Interleukin-6 (rHuIL-6) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Interleukin-6 (IL-6) is a 26-kD protein released by fibroblasts, T lymphocytes, endothelial cells, monocytes, and keratinocytes during inflammatory responses. IL-6 has pluripotent activities, including the stimulation of the monocytic lineage, the induction of megakaryopoiesis and the hepatic acute phase response, as well as the modulation of T- and B-cell responses. IL-6 is also capable of mediating direct and indirect tumor cell destruction and that it is potentially important for the immunotherapy of cancer[1. Recombinant human interleukin-6 (IL-6/BSF-2/HSF) regulates the synthesis of acute phase proteins in human hepatocytes[2]. |
