Recombinant Human Interleukin-5 是一種選擇性的人嗜酸性粒細胞功能激活劑。
Synonyms
rHuIL-5; EDF; BCDFII; TRF
Species
HumanSource
E. coli Accession
P05113 Gene ID
3567 Molecular Weight
Approximately 26.5 kDa AA Sequence
MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES Biological Activity
The ED50 is <1.0 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >1.0 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 25 mM Tris, pH 8.0. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Interleukin-5 (rHuIL-5) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Interleukin-5 (rHuIL-5) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Recombinant Human Interleukin-5 (rhIL-5) is a powerful and selective stimulator of human eosinophil function. IL-5 is thus the first hemopoietic factor whose elaboration in vivo can explain the selective eosinophilia and eosinophil activation seen in disease[1]. Recombinant human interleukin-5 (rhIL-5), in either liquid or semi- solid cultures, selectively induced eosinophil production from normal human bone marrow, with no activity on other cell lineages. Human interleukin-5 (IL-5) regulates the production of eosinophils in human bone marrowcultures: comparison and interaction with IL-1, IL-3, IL-6, and granulocyte-macrophage colony stimulating factor (GMCSF)[2]. |
