Recombinant Human Interleukin-33 是一種 Th2 細胞因子，是 ST2L 的特異性配體。
Synonyms
rHuIL-33; IL-1F11; NF-HEV; DVS 27
Species
HumanSource
E. coli Accession
O95760 Gene ID
90865 Molecular Weight
Approximately 18.0 kDa AA Sequence
SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET Biological Activity
The ED50 is <0.5 ng/mL as measured by D10S cells, corresponding to a specific activity of >2.0 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Interleukin-33 (rHuIL-33) to 100 μg/mL using ddH2. Storage & Stability
Lyophilized recombinant Human Interleukin-33 (rHuIL-33) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Human IL-33 is a proinflammatory protein that shares structural and functional characteristics with the IL-1 cytokine family. It binds and signals through the IL-1RL1/ST2 receptor activating NF-kappaB and MAP kinases. IL-33 induces production of Th2 cell related cytokines including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities. The IL-33-ST2 signaling cascade plays some roles in the pathophysiology of chronic allergic conjunctivitis through the activation of mast cells[1]. |
