Recombinant Human Interleukin-2 來源于E. coli，可用作治療結核病 (TB) 的輔助免疫治療劑。
Synonyms
rHuIL-2; IL2; T-cell Growth Factor; TCGF; Aldesleukin
Species
HumanSource
E. coli Accession
P60568 Gene ID
3558 Molecular Weight
Approximately 15,300 Da AA Sequence
MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFCQSIIS TLT Biological Activity
The ED50 is <0.1 ng/mL as measured by CTLL-2 cells, corresponding to a specific activity of >1 × 107 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 50mM Phosphate buffer, pH7.5, 5% Mannitol buffer. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Interleukin-2 (rHuIL-2) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Interleukin-2 (rHuIL-2) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Recombinant human interleukin-2 (IL-2) expressed as Escherichia coli is isolated as insoluble aggregates of protein (inclusion bodies) after cell breakage. Interleukin 2 (IL-2), a cytokine secreted by activated T cells, promotes the differentiation and proliferation of lymphoid cells as well as enhances the cell-mediated immune response to infections. Therefore IL-2 has been used as an adjunctive immunotherapeutic agent to control some infectious diseases, such as leishmaniasis, leprosy, and HIV infection[1]. |
