Recombinant Human Interleukin-1 Receptor Antagonist Protein是IL-1家族的成員，可與IL-1受體結合。
Synonyms
rHuIL-1RA; IL-1RN; IRAP
Species
HumanSource
E. coli Accession
P18510 Gene ID
3557 Molecular Weight
Approximately 17,258 Da AA Sequence
MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE Biological Activity
The ED50 is <0.5 ng/mL as measured by D10S cells, corresponding to a specific activity of 2.0 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 7.5, 200 mM NaCl buffer. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Interleukin-1 Receptor Antagonist Protein (rHuIL-1RA) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Interleukin-1 Receptor Antagonist Protein (rHuIL-1RA) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
The production of Human Interleukin-1 Receptor Antagonist Protein (IL-1ra) is stimulated by many substances including adherent IgG, other cytokines, and bacterial or viral components. Endogenous IL-1Ra is produced in numerous experimental animal models of disease as well as in human autoimmune and chronic inflammatory diseases. Treatment of human diseases with recombinant human IL-1Ra shows an absence of benefit in sepsis syndrome[1]. IL-1 receptor antagonist (IL-1ra) has provided a tool for blocking IL-1 activity in vivo, leading to a detailed understanding of the role of this cytokine in the inflammatory response[2]. |
