Recombinant Human Interferon-gamma 是一種細(xì)胞因子，具有免疫調(diào)節(jié)作用，可抗病毒、抗腫瘤等。
Synonyms
rHuIFN-γ; IFNG; IFN-gamma; Interferon gamma ; 重組人干擾素-γ
Species
HumanSource
E. coli Accession
P01579 Gene ID
3458 Molecular Weight
Approximately 17 kDa AA Sequence
MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ Biological Activity
The ED50 is <0.05 ng/mL as measured by HT‐29 cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Interferon-gamma (rHuIFN-γ) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Interferon-gamma (rHuIFN-γ) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Human Interferon-gamma (hIFNγ) is naturally produced by CD4+ T helper cell type 1 (Th1) lymphocytes, CD8+ cytotoxic lymphocytes, natural killer (NK) cells, B cells, NKT cells, and professional antigen-presenting cells (APCs). Secretion of hIFNγ by NK cells and APCs is important in early host reactions against infection while production of hIFNγ by T lymphocytes is important in the adaptive immune response. hIFNγ shows antiviral and antitumor activity and is involved in complex interactions of cellular metabolism and differentiation[1]. Interferon-gamma (IFN-γ) is a cytokine with potent immunomodulatory propertiy. IFN-γ activates cells via a different receptor than IFN-α and IFN-β, which accounts for the different physiological properties of the proteins[2]. |
