Recombinant Human Epigen (CHO-expressed) 是一種表皮生長因子，對只表達(dá) ErbB-2 的細(xì)胞沒有刺激作用，在表達(dá) ErbB-1， 共表達(dá) ErbB-2 和其他 ErbB 的細(xì)胞中，作為細(xì)胞分裂素能夠促進(jìn)細(xì)胞有絲分裂。
Synonyms
rHuEpigen; Epithelial mitogen; EPG ; 重組人 Epigen 因子 (CHO 細(xì)胞表達(dá))
Species
HumanSource
CHO Accession
Q6UW88 Gene ID
255324 Molecular Weight
15-20 kDa AA Sequence
AVTVTPPITA QQGNWTVNKT EADNIEGPIA LKFSHLCLED HNSYCINGAC AFHHELEKAI CRCFTGYTGE RCEHLTLTSY A Biological Activity
The ED50 is <1 μg/mL as measured by 3T3 cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, pH 7.4. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Epigen (CHO-expressed) (rHuEpigen) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Epigen (CHO-expressed) (rHuEpigen) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Recombinant Human Epigen (CHO-expressed) is a EGF-like growth factor that does not stimulate cells singly expressing ErbB-2, but acts as a mitogen for cells expressing ErbB-1 and co-expressing ErbB-2 in combination with the other ErbBs, with potent mitogenic activity[1]. |
